WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2181801
total bases: 314179344
Q20 bases: 305961140(97.3842%)
Q30 bases: 290020422(92.3105%)

Read1 after filtering:
total reads: 2181100
total bases: 313750581
Q20 bases: 305825978(97.4742%)
Q30 bases: 289957341(92.4165%)

Filtering result:
reads passed filter: 2181100
reads failed due to low quality: 229
reads failed due to too many N: 472
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 93.43%

JSON report: SewardAK_302.json
HTML report: SewardAK_302.html

fastp -i SewardAK_302.F.fq.gz -o SewardAK_302.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_302.json -h SewardAK_302.html 
fastp v0.20.0, time used: 29 seconds