WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2476073
total bases: 356554512
Q20 bases: 347142114(97.3602%)
Q30 bases: 328674931(92.1808%)

Read1 after filtering:
total reads: 2475196
total bases: 356251840
Q20 bases: 347022015(97.4092%)
Q30 bases: 328601774(92.2386%)

Filtering result:
reads passed filter: 2475196
reads failed due to low quality: 346
reads failed due to too many N: 531
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 92.8135%

JSON report: SewardAK_301.json
HTML report: SewardAK_301.html

fastp -i SewardAK_301.F.fq.gz -o SewardAK_301.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_301.json -h SewardAK_301.html 
fastp v0.20.0, time used: 32 seconds