WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3392382
total bases: 488503008
Q20 bases: 475127493(97.2619%)
Q30 bases: 449813573(92.08%)

Read1 after filtering:
total reads: 3391194
total bases: 487911333
Q20 bases: 474929606(97.3393%)
Q30 bases: 449714155(92.1713%)

Filtering result:
reads passed filter: 3391194
reads failed due to low quality: 473
reads failed due to too many N: 715
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 91.5769%

JSON report: SewardAK_300.json
HTML report: SewardAK_300.html

fastp -i SewardAK_300.F.fq.gz -o SewardAK_300.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_300.json -h SewardAK_300.html 
fastp v0.20.0, time used: 39 seconds