WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3075183
total bases: 442826352
Q20 bases: 434454083(98.1094%)
Q30 bases: 421323367(95.1441%)

Read1 after filtering:
total reads: 3071920
total bases: 442354446
Q20 bases: 434122115(98.139%)
Q30 bases: 421043158(95.1823%)

Filtering result:
reads passed filter: 3071920
reads failed due to low quality: 810
reads failed due to too many N: 2453
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.4743%

JSON report: SekiuWA_FG035.json
HTML report: SekiuWA_FG035.html

fastp -i SekiuWA_FG035.F.fq.gz -o SekiuWA_FG035.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG035.json -h SekiuWA_FG035.html 
fastp v0.20.0, time used: 34 seconds