WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 5081601
total bases: 731750544
Q20 bases: 717926607(98.1108%)
Q30 bases: 696158671(95.1361%)

Read1 after filtering:
total reads: 5076287
total bases: 730981246
Q20 bases: 717379374(98.1392%)
Q30 bases: 695696441(95.173%)

Filtering result:
reads passed filter: 5076287
reads failed due to low quality: 1188
reads failed due to too many N: 4126
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 80.2249%

JSON report: SekiuWA_FG033.json
HTML report: SekiuWA_FG033.html

fastp -i SekiuWA_FG033.F.fq.gz -o SekiuWA_FG033.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG033.json -h SekiuWA_FG033.html 
fastp v0.20.0, time used: 49 seconds