WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3547918
total bases: 510900192
Q20 bases: 501352601(98.1312%)
Q30 bases: 486349404(95.1946%)

Read1 after filtering:
total reads: 3544242
total bases: 510369049
Q20 bases: 500972578(98.1589%)
Q30 bases: 486026616(95.2304%)

Filtering result:
reads passed filter: 3544242
reads failed due to low quality: 764
reads failed due to too many N: 2912
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 85.3938%

JSON report: SekiuWA_FG032.json
HTML report: SekiuWA_FG032.html

fastp -i SekiuWA_FG032.F.fq.gz -o SekiuWA_FG032.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG032.json -h SekiuWA_FG032.html 
fastp v0.20.0, time used: 39 seconds