WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4904943
total bases: 706311792
Q20 bases: 693096380(98.129%)
Q30 bases: 672257261(95.1785%)

Read1 after filtering:
total reads: 4899966
total bases: 705591504
Q20 bases: 692582615(98.1563%)
Q30 bases: 671820495(95.2138%)

Filtering result:
reads passed filter: 4899966
reads failed due to low quality: 1051
reads failed due to too many N: 3926
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 81.7704%

JSON report: SekiuWA_FG027.json
HTML report: SekiuWA_FG027.html

fastp -i SekiuWA_FG027.F.fq.gz -o SekiuWA_FG027.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG027.json -h SekiuWA_FG027.html 
fastp v0.20.0, time used: 49 seconds