WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3321813
total bases: 478341072
Q20 bases: 469315561(98.1132%)
Q30 bases: 455044722(95.1298%)

Read1 after filtering:
total reads: 3318356
total bases: 477840889
Q20 bases: 468956665(98.1408%)
Q30 bases: 454739421(95.1654%)

Filtering result:
reads passed filter: 3318356
reads failed due to low quality: 688
reads failed due to too many N: 2769
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 85.2761%

JSON report: SekiuWA_FG026.json
HTML report: SekiuWA_FG026.html

fastp -i SekiuWA_FG026.F.fq.gz -o SekiuWA_FG026.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG026.json -h SekiuWA_FG026.html 
fastp v0.20.0, time used: 36 seconds