WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3828189
total bases: 551259216
Q20 bases: 540392169(98.0287%)
Q30 bases: 523651206(94.9918%)

Read1 after filtering:
total reads: 3824279
total bases: 550693801
Q20 bases: 539988605(98.0561%)
Q30 bases: 523307454(95.0269%)

Filtering result:
reads passed filter: 3824279
reads failed due to low quality: 845
reads failed due to too many N: 3065
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 84.28%

JSON report: SekiuWA_FG016.json
HTML report: SekiuWA_FG016.html

fastp -i SekiuWA_FG016.F.fq.gz -o SekiuWA_FG016.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG016.json -h SekiuWA_FG016.html 
fastp v0.20.0, time used: 41 seconds