WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3781678
total bases: 544561632
Q20 bases: 534331688(98.1214%)
Q30 bases: 518188416(95.157%)

Read1 after filtering:
total reads: 3777780
total bases: 543998305
Q20 bases: 533926686(98.1486%)
Q30 bases: 517843819(95.1922%)

Filtering result:
reads passed filter: 3777780
reads failed due to low quality: 785
reads failed due to too many N: 3113
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 85.1197%

JSON report: SekiuWA_FG015.json
HTML report: SekiuWA_FG015.html

fastp -i SekiuWA_FG015.F.fq.gz -o SekiuWA_FG015.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG015.json -h SekiuWA_FG015.html 
fastp v0.20.0, time used: 40 seconds