WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4316703
total bases: 621605232
Q20 bases: 609340057(98.0269%)
Q30 bases: 589983580(94.9129%)

Read1 after filtering:
total reads: 4312543
total bases: 621002972
Q20 bases: 608907706(98.0523%)
Q30 bases: 589617193(94.946%)

Filtering result:
reads passed filter: 4312543
reads failed due to low quality: 762
reads failed due to too many N: 3398
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 82.3669%

JSON report: SekiuWA_FG014.json
HTML report: SekiuWA_FG014.html

fastp -i SekiuWA_FG014.F.fq.gz -o SekiuWA_FG014.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG014.json -h SekiuWA_FG014.html 
fastp v0.20.0, time used: 46 seconds