WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4851746
total bases: 698651424
Q20 bases: 684904176(98.0323%)
Q30 bases: 663472127(94.9647%)

Read1 after filtering:
total reads: 4846887
total bases: 697948488
Q20 bases: 684402131(98.0591%)
Q30 bases: 663047225(94.9995%)

Filtering result:
reads passed filter: 4846887
reads failed due to low quality: 950
reads failed due to too many N: 3908
reads failed due to too short: 1
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 82.0794%

JSON report: SekiuWA_FG013.json
HTML report: SekiuWA_FG013.html

fastp -i SekiuWA_FG013.F.fq.gz -o SekiuWA_FG013.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG013.json -h SekiuWA_FG013.html 
fastp v0.20.0, time used: 49 seconds