WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4451823
total bases: 641062512
Q20 bases: 628314620(98.0114%)
Q30 bases: 608327087(94.8936%)

Read1 after filtering:
total reads: 4447439
total bases: 640426499
Q20 bases: 627856162(98.0372%)
Q30 bases: 607937835(94.927%)

Filtering result:
reads passed filter: 4447439
reads failed due to low quality: 858
reads failed due to too many N: 3526
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 82.9204%

JSON report: SekiuWA_FG005.json
HTML report: SekiuWA_FG005.html

fastp -i SekiuWA_FG005.F.fq.gz -o SekiuWA_FG005.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG005.json -h SekiuWA_FG005.html 
fastp v0.20.0, time used: 47 seconds