WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4158658
total bases: 598846752
Q20 bases: 587186206(98.0528%)
Q30 bases: 568848207(94.9906%)

Read1 after filtering:
total reads: 4154384
total bases: 598227943
Q20 bases: 586750498(98.0814%)
Q30 bases: 568482051(95.0277%)

Filtering result:
reads passed filter: 4154384
reads failed due to low quality: 1007
reads failed due to too many N: 3267
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 85.2458%

JSON report: SekiuWA_FG004.json
HTML report: SekiuWA_FG004.html

fastp -i SekiuWA_FG004.F.fq.gz -o SekiuWA_FG004.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG004.json -h SekiuWA_FG004.html 
fastp v0.20.0, time used: 42 seconds