WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4107369
total bases: 591461136
Q20 bases: 579840701(98.0353%)
Q30 bases: 561635365(94.9573%)

Read1 after filtering:
total reads: 4103345
total bases: 590879008
Q20 bases: 579421704(98.061%)
Q30 bases: 561277912(94.9903%)

Filtering result:
reads passed filter: 4103345
reads failed due to low quality: 762
reads failed due to too many N: 3262
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 83.2864%

JSON report: SekiuWA_FG003.json
HTML report: SekiuWA_FG003.html

fastp -i SekiuWA_FG003.F.fq.gz -o SekiuWA_FG003.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG003.json -h SekiuWA_FG003.html 
fastp v0.20.0, time used: 43 seconds