WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1400166
total bases: 201623904
Q20 bases: 195279526(96.8534%)
Q30 bases: 183426644(90.9747%)

Read1 after filtering:
total reads: 1399389
total bases: 201448592
Q20 bases: 195193203(96.8948%)
Q30 bases: 183367734(91.0246%)

Filtering result:
reads passed filter: 1399389
reads failed due to low quality: 378
reads failed due to too many N: 399
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 90.3704%

JSON report: SekiuWA_67.json
HTML report: SekiuWA_67.html

fastp -i SekiuWA_67.F.fq.gz -o SekiuWA_67.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_67.json -h SekiuWA_67.html 
fastp v0.20.0, time used: 22 seconds