WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2110098
total bases: 303854112
Q20 bases: 295070381(97.1092%)
Q30 bases: 277901277(91.4588%)

Read1 after filtering:
total reads: 2109182
total bases: 303616808
Q20 bases: 294958873(97.1484%)
Q30 bases: 277826701(91.5057%)

Filtering result:
reads passed filter: 2109182
reads failed due to low quality: 377
reads failed due to too many N: 539
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 89.2929%

JSON report: SekiuWA_66.json
HTML report: SekiuWA_66.html

fastp -i SekiuWA_66.F.fq.gz -o SekiuWA_66.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_66.json -h SekiuWA_66.html 
fastp v0.20.0, time used: 27 seconds