WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1831775
total bases: 263775600
Q20 bases: 255799956(96.9764%)
Q30 bases: 240615228(91.2197%)

Read1 after filtering:
total reads: 1830923
total bases: 263376581
Q20 bases: 255660607(97.0704%)
Q30 bases: 240544939(91.3312%)

Filtering result:
reads passed filter: 1830923
reads failed due to low quality: 342
reads failed due to too many N: 510
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.3712%

JSON report: SekiuWA_65.json
HTML report: SekiuWA_65.html

fastp -i SekiuWA_65.F.fq.gz -o SekiuWA_65.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_65.json -h SekiuWA_65.html 
fastp v0.20.0, time used: 25 seconds