WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2138537
total bases: 307949328
Q20 bases: 299084009(97.1212%)
Q30 bases: 281754545(91.4938%)

Read1 after filtering:
total reads: 2137425
total bases: 307561062
Q20 bases: 298931866(97.1943%)
Q30 bases: 281668264(91.5812%)

Filtering result:
reads passed filter: 2137425
reads failed due to low quality: 504
reads failed due to too many N: 608
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 88.4575%

JSON report: SekiuWA_62.json
HTML report: SekiuWA_62.html

fastp -i SekiuWA_62.F.fq.gz -o SekiuWA_62.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_62.json -h SekiuWA_62.html 
fastp v0.20.0, time used: 28 seconds