WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2706619
total bases: 389753136
Q20 bases: 378473739(97.106%)
Q30 bases: 356010698(91.3426%)

Read1 after filtering:
total reads: 2705056
total bases: 389414368
Q20 bases: 378302193(97.1464%)
Q30 bases: 355891199(91.3914%)

Filtering result:
reads passed filter: 2705056
reads failed due to low quality: 740
reads failed due to too many N: 823
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.3686%

JSON report: SekiuWA_61.json
HTML report: SekiuWA_61.html

fastp -i SekiuWA_61.F.fq.gz -o SekiuWA_61.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_61.json -h SekiuWA_61.html 
fastp v0.20.0, time used: 33 seconds