WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2446462
total bases: 352290528
Q20 bases: 342085075(97.1031%)
Q30 bases: 322020572(91.4077%)

Read1 after filtering:
total reads: 2445249
total bases: 351995880
Q20 bases: 341943214(97.1441%)
Q30 bases: 321924403(91.4569%)

Filtering result:
reads passed filter: 2445249
reads failed due to low quality: 521
reads failed due to too many N: 692
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.1046%

JSON report: SekiuWA_59.json
HTML report: SekiuWA_59.html

fastp -i SekiuWA_59.F.fq.gz -o SekiuWA_59.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_59.json -h SekiuWA_59.html 
fastp v0.20.0, time used: 32 seconds