WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1854472
total bases: 267043968
Q20 bases: 259046304(97.0051%)
Q30 bases: 243506514(91.1859%)

Read1 after filtering:
total reads: 1853467
total bases: 266808382
Q20 bases: 258932132(97.048%)
Q30 bases: 243429990(91.2378%)

Filtering result:
reads passed filter: 1853467
reads failed due to low quality: 463
reads failed due to too many N: 542
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 88.6313%

JSON report: SekiuWA_54.json
HTML report: SekiuWA_54.html

fastp -i SekiuWA_54.F.fq.gz -o SekiuWA_54.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_54.json -h SekiuWA_54.html 
fastp v0.20.0, time used: 26 seconds