WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2118246
total bases: 305027424
Q20 bases: 295877348(97.0002%)
Q30 bases: 278363468(91.2585%)

Read1 after filtering:
total reads: 2117176
total bases: 304615905
Q20 bases: 295722608(97.0805%)
Q30 bases: 278279375(91.3542%)

Filtering result:
reads passed filter: 2117176
reads failed due to low quality: 474
reads failed due to too many N: 596
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.8807%

JSON report: SekiuWA_53.json
HTML report: SekiuWA_53.html

fastp -i SekiuWA_53.F.fq.gz -o SekiuWA_53.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_53.json -h SekiuWA_53.html 
fastp v0.20.0, time used: 29 seconds