WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3204245
total bases: 461411280
Q20 bases: 447768552(97.0433%)
Q30 bases: 421305878(91.3081%)

Read1 after filtering:
total reads: 3202528
total bases: 460783288
Q20 bases: 447528545(97.1234%)
Q30 bases: 421172738(91.4036%)

Filtering result:
reads passed filter: 3202528
reads failed due to low quality: 798
reads failed due to too many N: 919
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 88.8403%

JSON report: SekiuWA_52.json
HTML report: SekiuWA_52.html

fastp -i SekiuWA_52.F.fq.gz -o SekiuWA_52.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_52.json -h SekiuWA_52.html 
fastp v0.20.0, time used: 39 seconds