WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2554384
total bases: 367831296
Q20 bases: 356769211(96.9926%)
Q30 bases: 335759718(91.2809%)

Read1 after filtering:
total reads: 2553077
total bases: 367443818
Q20 bases: 356604284(97.05%)
Q30 bases: 335658504(91.3496%)

Filtering result:
reads passed filter: 2553077
reads failed due to low quality: 601
reads failed due to too many N: 706
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 88.1998%

JSON report: SekiuWA_50.json
HTML report: SekiuWA_50.html

fastp -i SekiuWA_50.F.fq.gz -o SekiuWA_50.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_50.json -h SekiuWA_50.html 
fastp v0.20.0, time used: 34 seconds