WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2317351
total bases: 333698544
Q20 bases: 325097808(97.4226%)
Q30 bases: 307571785(92.1706%)

Read1 after filtering:
total reads: 2316394
total bases: 333431199
Q20 bases: 324979576(97.4653%)
Q30 bases: 307492916(92.2208%)

Filtering result:
reads passed filter: 2316394
reads failed due to low quality: 415
reads failed due to too many N: 542
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 74.9725%

JSON report: SekiuWA_49.json
HTML report: SekiuWA_49.html

fastp -i SekiuWA_49.F.fq.gz -o SekiuWA_49.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_49.json -h SekiuWA_49.html 
fastp v0.20.0, time used: 29 seconds