WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3367948
total bases: 484984512
Q20 bases: 470965377(97.1094%)
Q30 bases: 443322099(91.4095%)

Read1 after filtering:
total reads: 3366371
total bases: 484612854
Q20 bases: 470779409(97.1455%)
Q30 bases: 443192395(91.4529%)

Filtering result:
reads passed filter: 3366371
reads failed due to low quality: 630
reads failed due to too many N: 947
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.1772%

JSON report: SekiuWA_48.json
HTML report: SekiuWA_48.html

fastp -i SekiuWA_48.F.fq.gz -o SekiuWA_48.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_48.json -h SekiuWA_48.html 
fastp v0.20.0, time used: 40 seconds