WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3467494
total bases: 499319136
Q20 bases: 484376429(97.0074%)
Q30 bases: 455854997(91.2953%)

Read1 after filtering:
total reads: 3465750
total bases: 498823500
Q20 bases: 484160230(97.0604%)
Q30 bases: 455719803(91.3589%)

Filtering result:
reads passed filter: 3465750
reads failed due to low quality: 799
reads failed due to too many N: 945
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.3267%

JSON report: SekiuWA_46.json
HTML report: SekiuWA_46.html

fastp -i SekiuWA_46.F.fq.gz -o SekiuWA_46.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_46.json -h SekiuWA_46.html 
fastp v0.20.0, time used: 40 seconds