WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4131902
total bases: 594993888
Q20 bases: 577926111(97.1314%)
Q30 bases: 544205005(91.464%)

Read1 after filtering:
total reads: 4129929
total bases: 594241896
Q20 bases: 577637275(97.2057%)
Q30 bases: 544042315(91.5523%)

Filtering result:
reads passed filter: 4129929
reads failed due to low quality: 786
reads failed due to too many N: 1187
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 83.459%

JSON report: SekiuWA_44.json
HTML report: SekiuWA_44.html

fastp -i SekiuWA_44.F.fq.gz -o SekiuWA_44.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_44.json -h SekiuWA_44.html 
fastp v0.20.0, time used: 47 seconds