WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3572271
total bases: 514407024
Q20 bases: 499316453(97.0664%)
Q30 bases: 470216973(91.4095%)

Read1 after filtering:
total reads: 3570777
total bases: 513982854
Q20 bases: 499125650(97.1094%)
Q30 bases: 470092985(91.4608%)

Filtering result:
reads passed filter: 3570777
reads failed due to low quality: 571
reads failed due to too many N: 923
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.2031%

JSON report: SekiuWA_43.json
HTML report: SekiuWA_43.html

fastp -i SekiuWA_43.F.fq.gz -o SekiuWA_43.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_43.json -h SekiuWA_43.html 
fastp v0.20.0, time used: 39 seconds