WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2509364
total bases: 361348416
Q20 bases: 350487251(96.9943%)
Q30 bases: 329541258(91.1976%)

Read1 after filtering:
total reads: 2508135
total bases: 360860165
Q20 bases: 350304259(97.0748%)
Q30 bases: 329442043(91.2935%)

Filtering result:
reads passed filter: 2508135
reads failed due to low quality: 502
reads failed due to too many N: 727
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.8562%

JSON report: SekiuWA_41.json
HTML report: SekiuWA_41.html

fastp -i SekiuWA_41.F.fq.gz -o SekiuWA_41.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_41.json -h SekiuWA_41.html 
fastp v0.20.0, time used: 33 seconds