WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2376402
total bases: 342201888
Q20 bases: 332123707(97.0549%)
Q30 bases: 312299024(91.2616%)

Read1 after filtering:
total reads: 2375220
total bases: 341920678
Q20 bases: 331985572(97.0943%)
Q30 bases: 312203795(91.3088%)

Filtering result:
reads passed filter: 2375220
reads failed due to low quality: 500
reads failed due to too many N: 682
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 83.2847%

JSON report: SekiuWA_38.json
HTML report: SekiuWA_38.html

fastp -i SekiuWA_38.F.fq.gz -o SekiuWA_38.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_38.json -h SekiuWA_38.html 
fastp v0.20.0, time used: 32 seconds