WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2150462
total bases: 309666528
Q20 bases: 300515176(97.0448%)
Q30 bases: 282789299(91.3206%)

Read1 after filtering:
total reads: 2149374
total bases: 309358527
Q20 bases: 300380761(97.0979%)
Q30 bases: 282704679(91.3842%)

Filtering result:
reads passed filter: 2149374
reads failed due to low quality: 496
reads failed due to too many N: 592
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 88.5827%

JSON report: SekiuWA_36.json
HTML report: SekiuWA_36.html

fastp -i SekiuWA_36.F.fq.gz -o SekiuWA_36.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_36.json -h SekiuWA_36.html 
fastp v0.20.0, time used: 30 seconds