WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2065221
total bases: 297391824
Q20 bases: 288600458(97.0438%)
Q30 bases: 271672508(91.3517%)

Read1 after filtering:
total reads: 2064239
total bases: 296991873
Q20 bases: 288451869(97.1245%)
Q30 bases: 271591852(91.4476%)

Filtering result:
reads passed filter: 2064239
reads failed due to low quality: 402
reads failed due to too many N: 580
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 88.5688%

JSON report: PortGambleWA_FG198.json
HTML report: PortGambleWA_FG198.html

fastp -i PortGambleWA_FG198.F.fq.gz -o PortGambleWA_FG198.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG198.json -h PortGambleWA_FG198.html 
fastp v0.20.0, time used: 28 seconds