WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3055624
total bases: 440009856
Q20 bases: 426823909(97.0033%)
Q30 bases: 401168329(91.1726%)

Read1 after filtering:
total reads: 3054186
total bases: 439472980
Q20 bases: 426615634(97.0744%)
Q30 bases: 401050941(91.2572%)

Filtering result:
reads passed filter: 3054186
reads failed due to low quality: 568
reads failed due to too many N: 870
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.9283%

JSON report: PortGambleWA_FG197.json
HTML report: PortGambleWA_FG197.html

fastp -i PortGambleWA_FG197.F.fq.gz -o PortGambleWA_FG197.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG197.json -h PortGambleWA_FG197.html 
fastp v0.20.0, time used: 38 seconds