WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2431577
total bases: 350147088
Q20 bases: 340017207(97.107%)
Q30 bases: 320308510(91.4783%)

Read1 after filtering:
total reads: 2430567
total bases: 349870402
Q20 bases: 339888774(97.147%)
Q30 bases: 320222332(91.526%)

Filtering result:
reads passed filter: 2430567
reads failed due to low quality: 366
reads failed due to too many N: 644
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 89.869%

JSON report: PortGambleWA_FG196.json
HTML report: PortGambleWA_FG196.html

fastp -i PortGambleWA_FG196.F.fq.gz -o PortGambleWA_FG196.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG196.json -h PortGambleWA_FG196.html 
fastp v0.20.0, time used: 32 seconds