WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3458744
total bases: 498059136
Q20 bases: 483215064(97.0196%)
Q30 bases: 454501916(91.2546%)

Read1 after filtering:
total reads: 3457065
total bases: 497422918
Q20 bases: 482971521(97.0947%)
Q30 bases: 454366253(91.3441%)

Filtering result:
reads passed filter: 3457065
reads failed due to low quality: 705
reads failed due to too many N: 974
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 85.701%

JSON report: PortGambleWA_FG195.json
HTML report: PortGambleWA_FG195.html

fastp -i PortGambleWA_FG195.F.fq.gz -o PortGambleWA_FG195.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG195.json -h PortGambleWA_FG195.html 
fastp v0.20.0, time used: 41 seconds