WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2444606
total bases: 352023264
Q20 bases: 341821071(97.1018%)
Q30 bases: 321653221(91.3727%)

Read1 after filtering:
total reads: 2443542
total bases: 351758607
Q20 bases: 341690500(97.1378%)
Q30 bases: 321562360(91.4156%)

Filtering result:
reads passed filter: 2443542
reads failed due to low quality: 379
reads failed due to too many N: 685
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 84.9146%

JSON report: PortGambleWA_FG194.json
HTML report: PortGambleWA_FG194.html

fastp -i PortGambleWA_FG194.F.fq.gz -o PortGambleWA_FG194.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG194.json -h PortGambleWA_FG194.html 
fastp v0.20.0, time used: 31 seconds