WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 2377576 total bases: 342370944 Q20 bases: 332399250(97.0875%) Q30 bases: 312934803(91.4023%) Read1 after filtering: total reads: 2376362 total bases: 341938519 Q20 bases: 332226202(97.1596%) Q30 bases: 312833215(91.4881%) Filtering result: reads passed filter: 2376362 reads failed due to low quality: 464 reads failed due to too many N: 750 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 85.0365% JSON report: PortGambleWA_FG192.json HTML report: PortGambleWA_FG192.html fastp -i PortGambleWA_FG192.F.fq.gz -o PortGambleWA_FG192.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG192.json -h PortGambleWA_FG192.html fastp v0.20.0, time used: 31 seconds