WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4849032
total bases: 698260608
Q20 bases: 677132066(96.9741%)
Q30 bases: 636134091(91.1027%)

Read1 after filtering:
total reads: 4846372
total bases: 697353762
Q20 bases: 676776631(97.0493%)
Q30 bases: 635934020(91.1925%)

Filtering result:
reads passed filter: 4846372
reads failed due to low quality: 1268
reads failed due to too many N: 1392
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 83.071%

JSON report: PortGambleWA_FG191.json
HTML report: PortGambleWA_FG191.html

fastp -i PortGambleWA_FG191.F.fq.gz -o PortGambleWA_FG191.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG191.json -h PortGambleWA_FG191.html 
fastp v0.20.0, time used: 53 seconds