WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2533433
total bases: 364814352
Q20 bases: 354037825(97.046%)
Q30 bases: 333468272(91.4077%)

Read1 after filtering:
total reads: 2532361
total bases: 364502989
Q20 bases: 353898194(97.0906%)
Q30 bases: 333377096(91.4607%)

Filtering result:
reads passed filter: 2532361
reads failed due to low quality: 400
reads failed due to too many N: 672
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 89.4848%

JSON report: PortGambleWA_FG190.json
HTML report: PortGambleWA_FG190.html

fastp -i PortGambleWA_FG190.F.fq.gz -o PortGambleWA_FG190.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG190.json -h PortGambleWA_FG190.html 
fastp v0.20.0, time used: 32 seconds