WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2631746
total bases: 378971424
Q20 bases: 358172429(94.5117%)
Q30 bases: 327080143(86.3073%)

Read1 after filtering:
total reads: 2630340
total bases: 377992453
Q20 bases: 357919267(94.6895%)
Q30 bases: 327011855(86.5128%)

Filtering result:
reads passed filter: 2630340
reads failed due to low quality: 1041
reads failed due to too many N: 365
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 55.7464%

JSON report: PortGambleWA_FG189.json
HTML report: PortGambleWA_FG189.html

fastp -i PortGambleWA_FG189.F.fq.gz -o PortGambleWA_FG189.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG189.json -h PortGambleWA_FG189.html 
fastp v0.20.0, time used: 35 seconds