WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3904179
total bases: 562201776
Q20 bases: 545230842(96.9813%)
Q30 bases: 512418803(91.145%)

Read1 after filtering:
total reads: 3902262
total bases: 561664773
Q20 bases: 544991634(97.0315%)
Q30 bases: 512265811(91.2049%)

Filtering result:
reads passed filter: 3902262
reads failed due to low quality: 820
reads failed due to too many N: 1097
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 83.2428%

JSON report: PortGambleWA_FG187.json
HTML report: PortGambleWA_FG187.html

fastp -i PortGambleWA_FG187.F.fq.gz -o PortGambleWA_FG187.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG187.json -h PortGambleWA_FG187.html 
fastp v0.20.0, time used: 44 seconds