WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3598827
total bases: 518231088
Q20 bases: 502907092(97.043%)
Q30 bases: 472722775(91.2185%)

Read1 after filtering:
total reads: 3596887
total bases: 517717664
Q20 bases: 502676413(97.0947%)
Q30 bases: 472575187(91.2805%)

Filtering result:
reads passed filter: 3596887
reads failed due to low quality: 921
reads failed due to too many N: 1019
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 82.8696%

JSON report: PortGambleWA_FG186.json
HTML report: PortGambleWA_FG186.html

fastp -i PortGambleWA_FG186.F.fq.gz -o PortGambleWA_FG186.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG186.json -h PortGambleWA_FG186.html 
fastp v0.20.0, time used: 42 seconds