WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2854134
total bases: 410995296
Q20 bases: 398880101(97.0522%)
Q30 bases: 375522804(91.3691%)

Read1 after filtering:
total reads: 2852720
total bases: 410459319
Q20 bases: 398676846(97.1294%)
Q30 bases: 375410050(91.461%)

Filtering result:
reads passed filter: 2852720
reads failed due to low quality: 620
reads failed due to too many N: 794
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.0001%

JSON report: PortGambleWA_FG185.json
HTML report: PortGambleWA_FG185.html

fastp -i PortGambleWA_FG185.F.fq.gz -o PortGambleWA_FG185.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG185.json -h PortGambleWA_FG185.html 
fastp v0.20.0, time used: 36 seconds