WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3292113
total bases: 474064272
Q20 bases: 460188806(97.0731%)
Q30 bases: 433535555(91.4508%)

Read1 after filtering:
total reads: 3290487
total bases: 473593444
Q20 bases: 459981511(97.1258%)
Q30 bases: 433402783(91.5137%)

Filtering result:
reads passed filter: 3290487
reads failed due to low quality: 677
reads failed due to too many N: 949
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.2596%

JSON report: PortGambleWA_FG183.json
HTML report: PortGambleWA_FG183.html

fastp -i PortGambleWA_FG183.F.fq.gz -o PortGambleWA_FG183.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG183.json -h PortGambleWA_FG183.html 
fastp v0.20.0, time used: 39 seconds