WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2984409
total bases: 429754896
Q20 bases: 417095481(97.0543%)
Q30 bases: 392152076(91.2502%)

Read1 after filtering:
total reads: 2983020
total bases: 429413913
Q20 bases: 416930426(97.0929%)
Q30 bases: 392039780(91.2965%)

Filtering result:
reads passed filter: 2983020
reads failed due to low quality: 576
reads failed due to too many N: 813
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 88.2956%

JSON report: PortGambleWA_FG182.json
HTML report: PortGambleWA_FG182.html

fastp -i PortGambleWA_FG182.F.fq.gz -o PortGambleWA_FG182.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG182.json -h PortGambleWA_FG182.html 
fastp v0.20.0, time used: 36 seconds