WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3175658
total bases: 457294752
Q20 bases: 433079633(94.7047%)
Q30 bases: 396374974(86.6782%)

Read1 after filtering:
total reads: 3174356
total bases: 456045585
Q20 bases: 432777605(94.8979%)
Q30 bases: 396307705(86.9009%)

Filtering result:
reads passed filter: 3174356
reads failed due to low quality: 893
reads failed due to too many N: 409
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 60.0503%

JSON report: PortGambleWA_FG181.json
HTML report: PortGambleWA_FG181.html

fastp -i PortGambleWA_FG181.F.fq.gz -o PortGambleWA_FG181.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG181.json -h PortGambleWA_FG181.html 
fastp v0.20.0, time used: 39 seconds