WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2605629
total bases: 375210576
Q20 bases: 364507402(97.1474%)
Q30 bases: 344564411(91.8323%)

Read1 after filtering:
total reads: 2604538
total bases: 374562337
Q20 bases: 364310953(97.2631%)
Q30 bases: 344481572(91.9691%)

Filtering result:
reads passed filter: 2604538
reads failed due to low quality: 531
reads failed due to too many N: 560
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.7022%

JSON report: PortGambleWA_FG180.json
HTML report: PortGambleWA_FG180.html

fastp -i PortGambleWA_FG180.F.fq.gz -o PortGambleWA_FG180.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG180.json -h PortGambleWA_FG180.html 
fastp v0.20.0, time used: 31 seconds