WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1583511
total bases: 228025584
Q20 bases: 221391042(97.0904%)
Q30 bases: 208363862(91.3774%)

Read1 after filtering:
total reads: 1582735
total bases: 227741348
Q20 bases: 221280418(97.163%)
Q30 bases: 208301315(91.464%)

Filtering result:
reads passed filter: 1582735
reads failed due to low quality: 321
reads failed due to too many N: 455
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.9061%

JSON report: MontereyBayCA_449.json
HTML report: MontereyBayCA_449.html

fastp -i MontereyBayCA_449.F.fq.gz -o MontereyBayCA_449.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_449.json -h MontereyBayCA_449.html 
fastp v0.20.0, time used: 25 seconds